Pubblicazione scientifica

Integrative Proteomic and MicroRNA Analysis: Insights Into Mechanisms of Eyestalk Ablation-Induced Ovarian Maturation in the Swimming Crab Portunus trituberculatus

FILONE TECNOLOGICO	TEMA
economia circolare e sostenibilità

RIASSUNTO

Eyestalk ablation is the most common method to induce ovarian maturation in decapod crustacean aquaculture, but it jeopardizes broodstock survival and larvae production. It is important to understand the molecular basis underlying the maturation triggered by ablation and thereby develop an alternative measure for maturation manipulation. In this study, we investigate alterations of ovarian proteome and miRNA profile after ablation in a commercially important marine crab Portunus trituberculatus. Quantitative proteomic analysis using iTRAQ reveals that 163 proteins are differentially expressed following ablation, and modulation of methyl farnesoate metabolism and activation of calcium signaling may play important roles in the ovarian maturation induced by ablation. miRNA expression profiling identifies 31 miRNAs that show statistically significant changes. Integration of miRNA and proteome expression data with miRNA target prediction algorithms generates a potential regulatory network consisting of 26 miRNAs and 30 proteins linked by 71 possible functional associations. The miRNA-protein network analysis suggests that miRNAs are involved in promoting ovarian maturation by controlling expression of proteins related to methyl farnesoate synthesis, calcium signals, and energy metabolism. Experimental validation and temporal expression analysis indicate multiple miRNAs can act synergistically to regulate expression of Farnesoic acid O-methyltransferase and Calmodulin. Our findings provide new insights for elucidating the mechanisms underlying eyestalk ablation-induced ovarian maturation and could be useful for devising an alternative technique for manipulating reproduction in P. trituberculatus and other decapods.

DATA

Data di pubblicazione: 14/08/2020

AUTORI

MENG XIANLIANG
ZHANG MENGQIAN
GAO BAOQUAN
LV JIANJIAN
LI JIAN
LIU PING

ENTE DI AFFERENZA

KEY LAB OF AQUATIC GENOMICS MINST AGRICULTURE & RURAL AFFAIRS YELLOW SEA FISHERIES RSCH INST CHINESE ACAD FISHERY SCIENCES QINGDAO CHINA
LAB FOR MARINE FISHERIES SCIENCE & FOOD PROD PROCESSES QINGDAO NATL LAB FOR MARINE SCIENCE & TECH QINGDAO CHINA
KEY LAB OF SUSTAINABLE DEV OF MARINE FISHERIES MINST AGRICULTURE & RURAL AFFAIRS YELLOW SEA FISHERIES RSCH INST CHINESE ACAD FISHERY SCIENCES QINGDAO CHINA
COLLEGE OF MARINE LIFE & FISHERIES JIANGSU OCEAN UNIV LIANYUNGANG CHINA

RIVISTA

Frontiers in Endocrinology, 11, 533

IMPACT FACTOR

3,630

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