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Metagenomic profiling of historic Colorado Front Range flood impact on distribution of riverine antibiotic resistance genes

Record-breaking floods in September 2013 caused massive damage to homes and infrastructure across the Colorado Front Range and heavily impacted the Cache La Poudre River watershed. Given the unique nature of this watershed as a test-bed for tracking environmental pathways of antibiotic resistance gene ...

Regional variations in the diversity and predicted metabolic potential of benthic prokaryotes in coastal northern Zhejiang, East China Sea

Knowledge about the drivers of benthic prokaryotic diversity and metabolic potential in interconnected coastal sediments at regional scales is limited. We collected surface sediments across six zones covering ~200 km in coastal northern Zhejiang, East China Sea and combined 16 S rRNA gene sequencing, ...

The effects of light exposure during incubation on embryonic development and hatchling traits in lizards

Light is an environmental factor that is known to profoundly affect embryonic development in some oviparous vertebrates, but such effects are unstudied in reptiles. We investigated the light sensitivity of lizard embryos by examining the thickness and light transmittance of eggshells as well as the ...

Diverse Profiles of AI-1 Type Quorum Sensing Molecules in Cultivable Bacteria from the Mangrove (Kandelia obovata) Rhizosphere Environment

Mangrove rhizosphere environment harbors diverse populations of microbes, and some evidence showed that rhizobacteria behavior was regulated by quorum sensing (QS). Investigating the diverse profiles of QS molecules in mangrove ecosystems may shed light on the bacterial roles and lead to a better understanding ...

Pre-Messinian Petroleum Systems and Trap Style in the Offshore Western of Nile Delta; An Integrated Geological and Geophysical Approach

Abstract Exploration in the offshore Nile Delta province has revealed several hydrocarbon plays. Deep marine Turbidites is considered one of the most important plays for hydrocarbon exploration in the Nile Delta. These turbidites vary from submarine turbidite channels to submarine basin floor fans. An ...

Brownification increases winter mortality in fish

In northern climates, winter is a bottleneck for many organisms. Low light and resource availability constrains individual foraging rates, potentially leading to starvation and increased mortality. Increasing input of humic substances to aquatic ecosystems causes brownification of water and hence a ...

Antifreeze glycopeptides: from structure and activity studies to current approaches in chemical synthesis

Antifreeze glycopeptides (AFGPs) are a class of biological antifreeze agents found predominantly in Arctic and Antarctic species of fish. They possess the ability to regulate ice nucleation and ice crystal growth, thus creating viable life conditions at temperatures below the freezing point of body ...

Transcriptome Analysis of the Tadpole Shrimp (Triops longicaudatus) by Illumina Paired-End Sequencing: Assembly, Annotation, and Marker Discovery

The tadpole shrimp (Triops longicaudatus) is an aquatic crustacean that helps control pest populations. It inhabits freshwater ponds and pools and has been described as a living fossil. T. longicaudatus was officially declared an endangered species South Korea in 2005; however, through subsequent protection ...

Larval diet affects mosquito development and permissiveness to Plasmodium infection

The larval stages of malaria vector mosquitoes develop in water pools, feeding mostly on microorganisms and environmental detritus. Richness in the nutrient supply to larvae influences the development and metabolism of larvae and adults. Here, we investigated the effects of larval diet on the development, ...

Visually Grounded Meaning Representations

In this paper we address the problem of grounding distributional representations of lexical meaning. We introduce a new model which uses stacked autoencoders to learn higher-level representations from textual and visual input. The visual modality is encoded via vectors of attributes obtained automatically ...

Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads

A potentiometric aptasensing assay that couples the DNA nanostructure-modified magnetic beads with a solid-contact polycation-sensitive membrane electrode for the detection of Vibrio alginolyticus is herein described. The DNA nanostructure-modified magnetic beads are used for amplification of the potential ...

Genetic Diversity and Structure Analysis of Percocypris pingi (Cypriniformes: Cyprinidae): Implications for Conservation and Hatchery Release in the Yalong River

Percocypris pingi is a near threatened cyprinid species, which has suffered a dramatic decline due to anthropogenic factors. As one response to this decline, hatchery release for P. pingi has been conducted in the lower reaches of the Yalong River since 2012. To understand the conservation status of ...

A DNA vaccine encoding the viral hemorrhagic septicemia virus genotype IVb glycoprotein confers protection in muskellunge (Esox masquinongy), rainbow trout (Oncorhynchus ...

Background The viral hemorrhagic septicemia virus (VHSV) is one of the most serious fish pathogens. In 2003, a novel sublineage (genotype IVb) of this deadly virus emerged in the Great Lakes basin causing serious fish kills. We have previously demonstrated that a DNA plasmid (pcDNA), containing a cytomegalovirus ...

Engineering of a thermostable esterase Est816 to improve its quorum-quenching activity and the underlying structural basis

N-acyl-homoserine lactones (AHLs) are small diffusible molecules called autoinducers that mediate cell-to-cell communications. Enzymatic degradation of AHLs is a promising bio-control strategy known as quorum-quenching. To improve the quorum-quenching activity of a thermostable esterase Est816, which ...

A wearable device with inertial motion tracking and vibro-tactile feedback for aesthetic sport athletes Diving Coach Monitor

Research into the kinematic training of elite divers shows a demand for diver training using biomechanical feedback. With suitable inertial data and tactile feedback, divers and their coaches could better understand their dives and failure modes. This would help improve technique and reduce fatigue ...

Simple underwater monitoring of shallow water using a spherical camera mounted on a radio-controlled boat

During underwater monitoring observations in shallow water for coastal environmental conservation, the sea is not always clear and waves and current make the observational situation difficult. Underwater monitoring is still conducted mainly by human divers. Therefore, underwater robotics or remotely ...

Assessment of Wave-induced Fatigue Damage to Marine Pipelines in Intermediate Seas

Abstract The accurate assessment of fatigue damage is a crucial issue for the design of marine pipelines. In this study, we modified the approach of Zheng et al. (2007) to simulate the random wave elevations for the fatigue assessment of marine pipelines applied to intermediate seas, e.g., the Persian ...

Design of a Low Temperature Differential (LTD) Stirling Engine for Water Transportation

Low temperature differential (LTD) Stirling engines (SE) are useful devices when attempting to obtain useful work from low temperature heat sources. In an attempt to implement a renewable small scale LTD SE on a fishing boat, the required energy sources must operate on green energy. This research focuses ...

Penumbral tourism: Place-based disaster education via real-world disaster simulation

Disaster education should be diversified using information and communication technology (ICT). To this end, we developed game-based evacuation drills (GBED) as ICT-based disaster education (ICTDE) programs. In the GBEDs, a participant occasionally views digital materials on a portable device, e.g. tablet ...

Teaching sustainable energy course through real world case studies, projects and simulations

This paper describes current and on-going effort to bring the new context of sustainable energy education to undergraduate students at the National University of Singapore. The course aims to introduce the students to the concept of modern electrical energy systems with different types of sustainable ...

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